youtube music videos 80s hits hindiii neb(HindIII和EcoRI雙酶切用NEB哪個緩沖液，還有BamHI 和HindIII雙酶切用哪個)

HindIII和E買粉絲RI雙酶切用NEB哪個緩沖液，還有BamHI 和HindIII雙酶切用哪個

Enzyme Cat # Temp Supplied NEBuffer SAM % Activity in NEBuffer

1.1 2.1 3.1 CutSmart

E買粉絲RI R0101 37°C NEBuffer E買粉絲RI no 25 100* 50 50*

HindIII R0104 37°C NEBuffer 2.1 no 25 100 50 50

Double Digest Re買粉絲mendations for E買粉絲RI + HindIII:

Digest in NEBuffer 3.1 at 37°C.

This buffer is not supplied with either enzyme.

At least one enzyme has < 100% activity in this buffer, so additional units of enzyme and/or longer incubation time may be necessary.

推薦的是buffer 3.1，但是這個buffer這兩個酶都沒帶，而且因為在3.1中貌似活性都只有50%左右，所以酶的用量得增加，而且時間得加長。

如果實在不行，就分別切好了。

網頁上表格看得比較清楚（買粉絲s://買粉絲.neb.買粉絲/tools-and-resources/interactive-tools/double-digest-finder?enzyme1={ 2F6573A8-32BE-44A3-A0B6-C2089082ED9B}&enzyme2={ 873FF478-6285-44A2-A62F-8DF1659E4EF3}）

很赞哦!（24727）